Radiation inactivation studies of hepatic sinusoidal reduced glutathione transport system
نویسندگان
چکیده
منابع مشابه
Novel properties of hepatic canalicular reduced glutathione transport revealed by radiation inactivation.
Transport of GSH at the canalicular pole of hepatocytes occurs by a facilitative carrier and can account for ∼50% of total hepatocyte GSH efflux. A low-affinity unit with sigmoidal kinetics accounts for 90% of canalicular transport at physiological GSH concentrations. A low-capacity transporter with high affinity for GSH has also been reported. It is not known whether the same or different prot...
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Glutathione (GSH) plays a critical role in many cellular processes, including the metabolism and detoxification of oxidants, metals, and other reactive electrophilic compounds of both endogenous and exogenous origin. Because the liver is a major site of GSH and glutathione S-conjugate biosynthesis and export, significant effort has been devoted to characterizing liver cell sinusoidal and canali...
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The functional-unit molecular size of the Na+/bile acid cotransport system and the apparent target size of the bile-acid-binding proteins in brush-border membrane vesicles from rabbit ileum were determined by radiation inactivation with high-energy electrons. The size of the functional transporting unit for Na(+)-dependent taurocholate uptake was determined to 451 +/- 35 kDa, whereas an apparen...
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where N/No is the fractional survival of the spore colony-forming ability, V is the "target volume" parameter in appropriate units, I is the radiation dose, and n is an integer (Woese, 1958a, b; Donnelan and Morowitz, 1957). The second equation is derived on the assumption that the radiosensitive portion of the spore consists of n pairs of targets, and survival occurs so long as one member of e...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2000
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(00)00152-8